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Echelon 是脂質研究和脂質與蛋白質相互作用研究領域的*。脂質蛋白相互作用是眾多的細胞信號通路中高級調控*的。異常的細胞信號通路將會導致許多疾病,例如癌癥,炎癥,糖尿病和心血管疾病。數(shù)年來,Echelon一直是脂質研究信息,脂質信號分析和試劑技術的供應商。
高品質的合成磷脂,磷酸肌醇,鞘脂及其衍生物
脂質與蛋白質相互作用工具
脂質含量檢測試劑盒
脂質相關生物酶活性檢測,抑制劑篩選試劑盒
優(yōu)質的樣品檢測服務及客戶服務
脂質蛋白相互作用工具
Lipid Strips/Arrays
產品PIP StripsTM, PIP ArraysTM:
簡單蛋白質疊加實驗可以快速識別和定量脂質、蛋白質相互作用。
包括所有八種磷酸肌醇。
Echelon,P-6001脂類試紙
PIP Strips are 2 x 6 cm hydrophobic membranes that have been spotted with 100 pmol of all eight phosphoinositides and seven other biological important lipids. They are used to determine how your protein of interest interacts with one or more of these lipids in a simple protein-lipid overlay assay. Bound protein is then detected by an antibody against the protein or affinity tag.
PIP膜試紙條是2 x 6 cm的疏水膜,包含8種磷脂和7種其他重要的生物脂類,被用來確定你感興趣的蛋白質如何與一個或多個這些脂質在一個簡單的蛋白質脂質重疊分析。包含以下:
1.Lysophosphatidic acid (LPA) 溶血磷脂酸(LPA)
2.Lysophosphocholine (LPC) 溶血磷脂
3.Phosphatidylinositol (PtdIns) 磷脂酰肌醇
4.Phosphatidylinositol (3)-phosphate (PtdIns(3)P) 磷脂酰肌醇(3)-磷酸
5.Phosphatidylinositol (4)-phosphate (PtdIns(4)P) 磷脂酰肌醇(4)-磷酸
6.Phosphatidylinositol (5)-phosphate (PtdIns(5)P) 磷脂酰肌醇(5)-磷酸
7.Phosphatidylethanolamine (PE) 磷脂酰乙 醇 胺
8.Phosphatidylcholine (PC) 磷脂酰膽堿
9.Sphingosine 1-Phosphate (S1P) 1-磷酸鞘氨醇
10.Phosphatidylinositol(3,4)-bisphosphate (PtdIns(3,4)P2 ) 磷脂酰肌醇(3,4)-二磷酸
11.Phosphatidylinositol(3,5)-bisphosphate (PtdIns(3,5)P2) 磷脂酰肌醇(3,5)-二磷酸
12.Phosphatidylinositol(4,5)-bisphosphate (PtdIns(4,5)P2) 磷脂酰肌醇(4,5)-二磷酸
13.Phosphatidylinositol(3,4,5)-trisphosphate (PtdIns(3,4,5)P3) 磷脂酰肌醇(3,4,5)-三磷酸
14.Phosphatidic acid (PA) 磷脂酸
15.Phosphatidylserine (PS) 磷脂酰絲氨酸
另外
Each 10-pack of PIP Strips includes a free vial of the PI(4,5)P2 specific binding protein for use as a positive control.---貨號P-6001-2 規(guī)格10PK,包含G-4501 蛋白;
發(fā)表文獻:
1)Huang, S., L. Gao, et al. (2006). “Heterodimeric Capping Protein from Arabidopsis Is Regulated by Phosphatidic Acid.” Molecular Biology of the Cell 17(4): 1946-1958.
2)Jin, W., W.-P. Ge, et al. (2006). “Lipid Binding Regulates Synaptic Targeting of PICK1, AMPA Receptor Trafficking, and Synaptic Plasticity.” The Journal of Neuroscience 26(9): 2380-2390.
3)Xi, Q., G. J. T. Pauer, et al. (2005). “Tubby-like Protein 1 (TULP1) Interacts with F-actin in Photoreceptor Cells.” Investigative Ophthalmology & Visual Science 46(12): 4754-4761.
4)Sharma, V. P., V. Desmarais, et al. (2008). “Immunostaining evidence for PI(4,5)P2 localization at the leading edge of chemoattractant-stimulated HL-60 cells.” J Leukoc Biol 84(2): 440-7.
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